CD90 抗体
Quick Overview for CD90 抗体 (ABIN118258)
抗原
See all CD90 (THY1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognizes Rat and Mouse CD90, also known as Thy1.1. Since Thy1.1 is a monomorphic determinant in Rat but polymorphic in Mice, clone MRC OX-7 reacts with Thy1.1 Mice e.g. AKR and FVB Mice, but not Thy1.2 Mice such as CBA and BALB/c. Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic neurons, using fluorescence microscopy. This clone has been reported to induce glomerular nephritis in Wistar Rats.Aff. Const.:The affinity of the Fab' of MRC OX-7 for Rat Thy1 is 3 x 10e9m-1 and for Mouse Thy1.1 is 3 x 10e8m-1 (1)
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纯化方法
- Affinity Chromatography on Protein G
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内毒素水平
- Low Endotoxin
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免疫原
- Rat Thy1 antigen. Remarks: Spleen cells from an immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
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亚型
- IgG1
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应用备注
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Flow Cytometry: 1/40 - 1/80, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.2 without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- CD90 (THY1)
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别名
- CD90
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背景
- Thy1.1 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (Mouse), immature B cells (Rat) and connective tissues.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
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基因ID
- 21838
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NCBI登录号
- NP_033408
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UniProt
- P01831
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途径
- Cell-Cell Junction Organization
抗原
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